{"id":4344,"date":"2012-02-21T11:20:31","date_gmt":"2012-02-21T19:20:31","guid":{"rendered":"http:\/\/www.functionalps.com\/blog\/?p=4344"},"modified":"2012-02-21T11:20:31","modified_gmt":"2012-02-21T19:20:31","slug":"fish-elongase-and-desaturase-enzymes","status":"publish","type":"post","link":"https:\/\/www.functionalps.com\/blog\/2012\/02\/21\/fish-elongase-and-desaturase-enzymes\/","title":{"rendered":"Fish &#8211; Elongase and Desaturase Enzymes"},"content":{"rendered":"<p>Aquaculture Volume 290, Issues 1\u20132, 4 May 2009, Pages 122\u2013131<br \/>\n<strong><a href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0044848609001392\">Physiological roles of fatty acyl desaturases and elongases in marine fish: Characterisation of cDNAs of fatty acyl \u03946 desaturase and elovl5 elongase of cobia (Rachycentron canadum)<\/a><\/strong><br \/>\n Xiaozhong Zheng, Zhaokun Ding, Youqing Xu, Oscar Monroig, Sofia Morais, Douglas R. Tocher<br \/>\nIn the present paper, we investigated the expression of fatty acyl desaturase and elongase genes in a marine teleost, cobia, a species of great interest due to its considerable aquaculture potential. A cDNA was cloned that, when expressed in yeast, was shown to result in desaturation of 18:3n\u22123 and 18:2n\u22126, indicating that it coded for a \u03946 desaturase enzyme. Very low desaturation of 20:4n\u22123 and 20:3n\u22126 indicated only trace \u03945 activity. Another cloned cDNA enabled elongation of 18:4n\u22123, 18:3n\u22126, 20:5n\u22123 and 20:4n\u22126 in the yeast expression system, indicating that it had C18\u201320 and C20\u201322 elongase activity. Sequence comparison and phylogenetic analysis confirmed that it was homologous to human ELOVL5 elongase. However, the cobia Elovl5 elongase also had low activity toward C24 HUFA. The cobia \u03946 desaturase had a preference for 18:3n\u22123, but the elongase was generally equally active with both n\u22123 and n\u22126 substrates. Expression of both genes was 1\u20132 orders of magnitude greater in brain than other tissues suggesting an important role, possibly to ensure sufficient docosahexaenoic acid (DHA, 22:6n\u22123) synthesis in neural tissues through elongation and desaturation of eicosapentaenoic acid (EPA; 20:5n\u22123).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Aquaculture Volume 290, Issues 1\u20132, 4 May 2009, Pages 122\u2013131 Physiological roles of fatty acyl desaturases and elongases in marine fish: Characterisation of cDNAs of fatty acyl \u03946 desaturase and elovl5 elongase of cobia (Rachycentron canadum) Xiaozhong Zheng, Zhaokun Ding, Youqing Xu, Oscar Monroig, Sofia Morais, Douglas R. Tocher In the present paper, we investigated [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[1192,649,1191,1193,650,98,647,363,496],"class_list":["post-4344","post","type-post","status-publish","format-standard","hentry","category-general","tag-desaturase","tag-dha","tag-double-bonds","tag-elongase","tag-epa","tag-fish-oil","tag-lipid-peroxides","tag-pufa","tag-unsaturated-fats"],"_links":{"self":[{"href":"https:\/\/www.functionalps.com\/blog\/wp-json\/wp\/v2\/posts\/4344","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.functionalps.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.functionalps.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.functionalps.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.functionalps.com\/blog\/wp-json\/wp\/v2\/comments?post=4344"}],"version-history":[{"count":1,"href":"https:\/\/www.functionalps.com\/blog\/wp-json\/wp\/v2\/posts\/4344\/revisions"}],"predecessor-version":[{"id":4345,"href":"https:\/\/www.functionalps.com\/blog\/wp-json\/wp\/v2\/posts\/4344\/revisions\/4345"}],"wp:attachment":[{"href":"https:\/\/www.functionalps.com\/blog\/wp-json\/wp\/v2\/media?parent=4344"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.functionalps.com\/blog\/wp-json\/wp\/v2\/categories?post=4344"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.functionalps.com\/blog\/wp-json\/wp\/v2\/tags?post=4344"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}